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Figure 1 | BMC Biochemistry

Figure 1

From: Autophosphorylation of serine 608 in the p85 regulatory subunit of wild type or cancer-associated mutants of phosphoinositide 3-kinase does not affect its lipid kinase activity

Figure 1

Characterisation of purified, recombinant PI3Ks. A. SDS-PAGE and Coomassie Blue staining of molecular weight standards (Fermentas, Lane 1), 0.5 μg purified p110αEEWT/p85α (lane 2), 0.5 μg purified p110αEEE545K/p85α (lane 3) and 0.5 μg purified p110αEEH1047R/p85α (lane4). B. Purified, recombinant PI3Ks were assayed for lipid kinase activity with increasing concentrations of ATP using PI-(4,5)-P2 as a substrate. Reactions were stopped after 20 minutes using 1 M HCl. The amount of 32P-PI-(3,4,5)-P3 generated was quantified using a phosphorimager and displayed as mean ± SEM, n = 4.

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