Figure 2

Purification and IgE reactivity of Aca s 4. The whole body extracts (20 μg) of A. siro (A.s WB) and D. farinae (D.f. WB) and the purified Aca s 4 (2.5 μg) were resolved by SDS-PAGE. Left-hand panel: A gel stained for protein with Coomassie blue. Right-hand panel: Western blot probed with pooled sera from mite-allergic patients sensitive to Dermatophagoides spp. and with anti-IgE antibodies and developed by chemiluminescence. For immunostaining inhibition (inhib.), the pooled sera were preincubated with purified Aca s 4. The arrows mark the position of Aca s 4 (~56 kDa) with the N-terminal sequence determined by Edman sequencing. Molecular mass standards are indicated.