Figure 5

Fibronectin levels are increased in microvascular preparations from PS1−/− embryonic brain. Microvascular preparations were collected on nylon filters from brains of the indicated embryonic (e) ages. “neur” indicates the “neuronal” fraction that passed through the nylon filer which was collected by centrifugation and then lysed for blotting. Blots were sequentially probed for fibronectin (FN) and β-tubulin (β-tub). For comparison primary endothelial cell cultures (EC) were prepared and allowed to expand for two weeks before being cultured in serum free growth media overnight and then harvested. 10 μg of protein was loaded per lane. A representative blot is shown from experiments that were performed three times.