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Figure 4 | BMC Biochemistry

Figure 4

From: Presenilin-1 regulates the constitutive turnover of the fibronectin matrix in endothelial cells

Figure 4

Increased levels of fibronectin in PS1−/− endothelial cells by Western blotting. Primary cultures of wild type and PS1−/− endothelial cells were grown to confluency in complete growth media containing fibronectin-depleted serum. The cells were harvested after washing with PBS and total cell lysates were prepared. Western blotting was performed using an anti-fibronectin (FN) antibody (upper panel in A). The middle panel shows the blot reprobed for β-tubulin (β-tub) as a loading control. The lowest panel in A shows the extracts probed with the antibody 33B10 which recognizes the C-terminal fragment (CTF) of PS1 to confirm the lack of detectible PS1 expression in PS1−/− endothelial cells. Panel B shows quantitation of the levels of fibronectin in the experiment shown in panel A. Asterisk indicates p < 0.05 vs. wild type (unpaired t-test). In panel C, passage 38 wild type or PS1−/− endothelial cells were cultured as above overnight. Total cell lysates were prepared and Western blotting was performed using an anti-fibronectin antibody (upper panel). Lower panel shows the blot reprobed for β-tubulin. All lanes were loaded with 10 μg of total protein. Representative blots are shown from experiments that were independently replicated three times.

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