Figure 12
From: Presenilin-1 regulates the constitutive turnover of the fibronectin matrix in endothelial cells
Delayed turnover of the fibronectin matrix in PS1−/− endothelial cells. Endothelial cells (p44) were pulsed with biotinylated fibronectin overnight and then switched to FN depleted medium and chased for the indicated times (hrs). At each time point the DOC soluble and insoluble fractions were prepared. One μg (insoluble) or 4 μg (soluble) samples were analyzed for biotinylated-FN by Western blotting using streptavidin-HRP. Samples were probed for vimentin (vim) and β-tubulin (β-tub) by Western blotting as controls for extraction of the insoluble and soluble fractions. Two independent replicates are shown.