Figure 11
From: Presenilin-1 regulates the constitutive turnover of the fibronectin matrix in endothelial cells
Increased levels of DOC insoluble biotinylated fibronectin following loading of PS1−/− endothelial cells. Passage 2 cultures of wild type (wt) and PS1−/− endothelial cells were grown in fibronectin-depleted serum and were loaded with biotinylated fibronectin (Biot-FN: 20 μg/ml) for the indicated times (hours). DOC-soluble and insoluble fractions were prepared and analyzed by Western blot. The membranes were probed with peroxidase conjugated-streptavidin and the DOC insoluble fraction was reprobed for vimentin (vim) while the DOC soluble fraction was reprobed for β-tubulin (β-tub). 1 μg of protein was loaded per lane.