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Figure 7 | BMC Biochemistry

Figure 7

From: RETRACTED ARTICLE: Proteomic analysis on N, N′-dinitrosopiperazine-mediated metastasis of nasopharyngeal carcinoma 6-10B cells

Figure 7

DNP-mediated 6-10B cell motility through AGR2. A, 6-10B cells were treated with the indicated concentration DNP for dose-course, and treated with 10 μM DNP for the indicated time for time-course. AGR2 expression in the DNP-treated cells was detected using Western-blotting. 6-10B cells (2 × 106) were transiently transfected with si-AGR2 or si-mock, incubated at 37°C for 16–24 h. Confluent monolayer of the transfect cells was then wounded using a plastic tip, and then treated with 10 μM DNP. (a) 6-10B cells treated with 0.1% DMSO. (b) 6-10B cells treated with 10 μM DNP. (c) The transfect with AGR2 si-RNA treated with DMSO. (d) The transfect treated with DNP. B, AGR2 expression in the above transfect cells with DMSO or DNP treatment using western-blotting. C, The treated cells were observed under microscope, and the cells migrating cross the boundaries lines in the center of the wells were counted. D, Numbers of cells that moved cross the lines (10 fields). The data are represented as the mean ± SD from three independent experiments. Results were statistically analyzed using one-way analysis of variance (ANOVA) with a post hoc Dunnett’s test (* p < 0.05). The error bars represent SDs. Scale bar, 10 μM. Arrow, cells moving cross the boundary.

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