Figure 6

Differential protein expression confirmed using Western blotting. A, eight representative proteins, aldo-keto reductase (AKR) 1B10, clusterin, cathepsin B, cathepsin D, ferritin, α-E-catenin, AGR2, and S100P were detected in 6-10B cells with or without DNP treatment using Western blotting. GAPDH served as a loading control. The relative photographic density was quantitated using a gel documentation and analysis system. B, AKR1B10, Cathepsin B and Clusterin were detected in the cell culture supernatant of 6-10B cells with or without DNP treatment using Western blotting. WB, Western blotting. SILAC, stable isotope labeling with amino acids in cell culture.