Figure 3
DNP-mediated 6-10B cell invasion and motility in vitro or metastasis in vivo. A, stable isotope-labeled 6-10B cells were treated with the indicated DNP concentration for 24 h. The treated cells were subjected to analyses for motility and invasion as described in the Materials and Methods. A, invasion of 6-10B cells at various concentrations. Arrow, invaded cell. B, motility of 6-10B cells at various concentrations; Arrow, motile cell. The data were statistically analyzed by one-way analysis of variance with post hoc Dunnett’s test (*, p < 0.05). Scale bar, 5 μM. C, twenty nude mice were injected with 6-10B cells in Matrigel through the tail vein (1 × 104 cells/mouse), and then randomly divided into two groups with 10 mice per group. The DNP group was abdominally injected with DNP using a stumped needle at a dosage of 40 mg/kg (bodyweight), twice a week for 60 days. The control was injected with 0.1% DMSO. After 60 days, tumor metastatic foci of 6-10B cells were observed. Scale bar, 10 μM. Arrow, tumor metastatic foci.