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Figure 2 | BMC Biochemistry

Figure 2

From: RETRACTED ARTICLE: Proteomic analysis on N, N′-dinitrosopiperazine-mediated metastasis of nasopharyngeal carcinoma 6-10B cells

Figure 2

Labeling of 6-10B cells with 2 H 4 -L-lysine and 13 C 615 N 4 -L-arginine. 6-10B cells were cultured in DMEM containing 2H4-L-lysine and 13C615N4-L-arginine or conventional L-lysine and L-arginine as described in the Materials and Methods. A, the peptide VEVTEFEDIK has a charge state of 1+ and contains one lysine; B, the peptide GHYTEGAELVDSVLDVVR has a charge state of 1+ and contains one arginine. C, the peptide LRQPFFQK has a charge state of 1+ and contains one lysine and one arginine. The peaks marked with blue and red represent the light isotope signal and the heavy isotope signal, respectively. Representative MS/MS and MS spectra from clusterin (D) and AKR1B10 (E). The inset shows the relative ratio of heavy to light versions of each precursor ion.

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