CPT1c KO mice and metabolomic profiling. (A) CPT1c protein from homogenized brains of WT and CPT1c KO mice were analyzed by western blot using the anti-CPT1c antibody. Hsc70 and Actin were used for loading controls. (B) A schematic pathway of metabolomic profiling for KO and WT brains. A commercial supplier of metabolic analysis homogenized 8 brain samples from independent mice to extract organic metabolites for performing unbiased metabolomic analysis using a mixture of GC-MS and LC-MS/MS.