Figure 1

Physical interaction between PRL-3 and integrin β1. (A) The expression profiles of integrin β1, integrin α1 and PRL-3 in different cell lines. Equal amount of protein lysates from indicated cells were subjected to Western blotting with integrin β1, integrin α1and PRL-3 antibodies. GAPDH protein expression was shown as loading control. (B) Endogenous interaction between PRL-3 and integrin β1. 500 μg of lysates from BGC823 (left panel) or SW480 (right panel) cells were immunoprecipitated with 1 μg PRL-3 monoclonal antibody or 1 μg pre-immune mouse IgG (control), followed by Western blotting with integrin β1 and PRL-3 antibodies. Expression of integrin β1 and PRL-3 in the lysates (50 μg) was shown as input. (C) Direct interaction between PRL-3 and integrin β1. GST-integrin β1 (intracellular domain, 752-798aa) and His-tagged PRL-3 were expressed respectively and used for in vitro pull-down assay.