Figure 7
Enrichment of antibodies for certain oxidized MRPs using affinity columns with other MRPs. (A) Western blot analysis of as-isolated and oxidized (10 mM H2O2, 12 h) MRP4 and MRP6 with antiserum against oxidized MRP3. “R” indicates as-isolated (i.e., reduced) protein, and “O” indicates oxidized protein. (B) Polyclonal antiserum against MRP3 was first purified on a protein G column, then fractionated on an affinity column with as-isolated MRP4, and the unbound fraction was further purified on a column with oxidized MRP4. The bound fraction from the latter column was used for detection of MRP4 oxidized under various H2O2 treatment conditions.