Skip to main content
Figure 2 | BMC Biochemistry

Figure 2

From: Characterization of methionine oxidation and methionine sulfoxide reduction using methionine-rich cysteine-free proteins

Figure 2

Analyses of Met oxidation and MetO reduction in MRPs by SDS-PAGE. (A) 60 μM MRP1 was treated with indicated concentrations of H2O2 for 7 or 12 h, and protein oxidation was monitored by the mobility shift on SDS-PAGE gels, as visualized by Coomassie Blue staining. Open triangles represent increasing treatment time (i.e., 7, 12 h). (B) 60 μM MRP5 was oxidized with indicated concentrations of H2O2 at room temperature for 12 h, and MetO formation was monitored by the mobility shift on SDS-PAGE gels, as visualized by Coomassie Blue staining. (C) Mass-spectrometry analysis of reduced MRP5 and the protein oxidized by 10 mM H2O2 at room temperature for 12 h. Theoretical and observed masses are shown.

Back to article page