Table 1 Kinetic parameters for the hydrolysis of p-NP-Neu5Ac by the pneumococcal sialidases computed using the direct and the indirect spectrophotometric assays (see Materials and Methods for details)
p- NP-Neu5Ac direct method | p-NP-Neu5Ac indirect method | |||||
|---|---|---|---|---|---|---|
k cat (s -1) | K m (μM) | kcat/K m (M -1·s -1) | k cat (s -1) | K m (μM) | kcat/K m (M -1·s -1) | |
NanA* | >175 | >500 | (3.5 ± 0.3) × 105 | >490 | >1,100 | (8.4 ± 0.9) × 104 |
NanB* | >0.14 | >500 | (2.7 ± 0.3) × 102 | >11 | >1,200 | (1.1 ± 0.1) × 102 |
NanC* | >17 | >500 | (3.4 ± 0.3) × 104 | >11 | >700 | (1.2 ± 0.1) × 104 |