Figure 4From: Optimization of a direct spectrophotometric method to investigate the kinetics and inhibition of sialidasesInhibition of NanA by the sialidase inhibitor Neu5Ac2en. The graph shows the dependence of the (Vmax/K m )/(Vmax/K m *) ratio as a function of the inhibitor concentration. The Vmax/Km* values were computed from the complete time-course reactions recorded in the presence of increasing concentrations of inhibitor and using 232 μM p- NP-Neu5Ac as the reporter substrate.Back to article page