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Figure 3 | BMC Biochemistry

Figure 3

From: Optimization of a direct spectrophotometric method to investigate the kinetics and inhibition of sialidases

Figure 3

Henri-Michaelis-Menten plot for the hydrolysis of p- NP-Neu5Ac by NanA. The normalized initial rates (V0/E0) are plotted as a function of substrate concentration, showing the perfect agreement between the initial rates measured with the conventional indirect method (empty circles) and our direct method (black circles). Reactions followed a first-order kinetics. Inset. An example of complete hydrolysis time course of 116, 232 and 464 μM p- NP-Neu5Ac (dotted, dashed and plain line, respectively) by 100 nM NanA, which allowed to compute the catalytic efficiency as described by DeMeester et al.[18]

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