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Table 2 Sequenced IGFP replacements that disrupted (clones 1 to 12) or not (clones 13 to 15) RicA-Rab2 interaction

From: Structural analysis of Brucella abortus RicA substitutions that do not impair interaction with human Rab2 GTPase

Clone n° X70GX72P sequence Y2H assay with Rab2
1 Y GG P His-, lacZ-
2 W GV P His-, lacZ-
3 T GV P His-, lacZ-
4 R GG P His-, lacZ-
5 A GH P His-, lacZ-
6 V GN P His-, lacZ-
7 L GE P His-, lacZ-
8 S G* P His-, lacZ-
9 A GS P His-, lacZ-
10 L GW P His-, lacZ-
11 L GH P His-, lacZ-
12 A GY P His-, lacZ-
13 S GF P His+, lacZ+
14 V GF P His+, lacZ+
15 K GL P His+, lacZ+
  1. The * indicates a stop codon. His- and His+ refer to the phenotype generated by the HIS3 reporter, and LacZ- and LacZ+ to the phenotype generated by the lacZ reporter.