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Table 2 Sequenced IGFP replacements that disrupted (clones 1 to 12) or not (clones 13 to 15) RicA-Rab2 interaction

From: Structural analysis of Brucella abortus RicA substitutions that do not impair interaction with human Rab2 GTPase

Clone n°

X70GX72P sequence

Y2H assay with Rab2

1

Y GG P

His-, lacZ-

2

W GV P

His-, lacZ-

3

T GV P

His-, lacZ-

4

R GG P

His-, lacZ-

5

A GH P

His-, lacZ-

6

V GN P

His-, lacZ-

7

L GE P

His-, lacZ-

8

S G* P

His-, lacZ-

9

A GS P

His-, lacZ-

10

L GW P

His-, lacZ-

11

L GH P

His-, lacZ-

12

A GY P

His-, lacZ-

13

S GF P

His+, lacZ+

14

V GF P

His+, lacZ+

15

K GL P

His+, lacZ+

  1. The * indicates a stop codon. His- and His+ refer to the phenotype generated by the HIS3 reporter, and LacZ- and LacZ+ to the phenotype generated by the lacZ reporter.