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Table 1 Distribution of substitutions in the mutated RicA clones interacting with Rab2 in a Y2H assay

From: Structural analysis of Brucella abortus RicA substitutions that do not impair interaction with human Rab2 GTPase

Clone n°

Substitutions (and their score in the Blosum62 matrix)

1, 12

T97S (1)

2

G8E (−2), G38S (0), D148V (−3)

3

N132Y (−2), R144C (−3)

4

V151I (3)

5

P2S (−1), D24H (−1), M101T (−1), V135A (0)

6

M127T (−1), E163D (2)

7

I65T (−1)

8

T121A (0)

9

I99N (−3), H166R (0)

10

P2S (−1), D24H (−1), I76N (−3), M101T (−1), V135A (0)

11

K10M (−1), P51T (−1)

13

M127V (−1)

14

E17G (−2), K124E (1)

15

I21V (3), E128V (−2), L143Q (−2)

16

H166R (0)

17

F13N (−3)

18

F39V (−1)

19

S157T (1)

20

M66L (2)

21

L45M (2), A119V (0)

22

I129V (3)

23

N7Y (−2)

24

S167T (1)

25

K30N (0)

26

Q12R (1)

28

K10E (1), M172L (2)

29

P2Q (−1)

31

T92S (1), A150T (0), G171C (−3)

32

I87T (−1)

  1. Clones 27 and 30 did not contain substitutions. Clones 1 and 12 contained the same substitutions.'