From: The role of the C8 proton of ATP in the catalysis of shikimate kinase and adenylate kinase
Enzyme | Km (mM) | v max | Kcat (s -1) | Kcat/Km (s-1 M -1) |
---|---|---|---|---|
1. SK WT ATP (M-M) | 6.533 6.53 ± 3.25 | 0.0484 233.8 ± 60.58 | 4.841 × 103 | 8.749 × 102 |
2. SK WT d-ATP (M-M) | 12.74 19.26 ± 7.00 | 0.0503 333.4 ± 89.2 | 5.027 × 103 | 3.946 × 102 |
3. SK-T17I ATP (A-S) | Ambig 30.73 ± 2.17 | Ambig 43.94 ± 3.87 | ||
4. SK-T17I d-ATP (M-M) | 22.05 ± 10.47 6.22 ± 3.57 | 0.0333 25.03 ± 7.70 | 1.331 × 103 | 60.06 |
5. SK-K15I ATP (M-M) | 4.054 4.13 ± 1.72 | 0.0057 2.736 ± 0.054 | 5.664 × 10 | 1.397 × 10 |
6. SK-K15I d-ATP (A-S) | 1.091 0.754 ± 0.227 | 0.0023 0.971 ± 0.031 | 2.369 × 10 | 2.171 × 10 |
7. AK1 WT ATP1 (A-S) | 1.911 | 0.00219 | 6.649 × 103 | 3.479 × 103 |
0.2332 | 141.7 | |||
8. AK1 WT d-ATP (M-M) | 4.043 | 0.00250 | 7.583 × 103 | 1.875 × 103 |
8.404 | 557.7 | |||
9. AK1-T23V ATP (M-M) | 12.91 | 0.0148 | 4.497 × 104 | 3.483 × 103 |
12.91 | 1850 | |||
10. AK1-T23V d-ATP (M-M) | 10.51 | 0.00963 | 2.919 × 104 | 2.777 × 103 |
.22 | 1062 | |||
11. AK1-R128A ATP | Ambig | Ambig 4.597 | Ambig | |
59.90 | ||||
12. AK1-R128A d-ATP | Ambig | Ambig | ||
13.36 | 1.299 | |||
13. AK1-T39V ATP (A-S) | 5.485 | 0.00398 | 1.989 × 103 | 3.626 × 102 |
1.191 | 29.57 | |||
14. AK1-T39V d-ATP (M-M) | 3.235 | 0.00159 | 7.972 × 102 | 2.464 × 102 |
4.040 | 39.21 | |||
15. AK1-R97A ATP (A-S) | 0.1226 | 0.00131 2.532 | 6.527 × 102 | 5.323 × 103 |
0.0002 | ||||
16. AK1-R97A d-ATP (M-M) | Ambig | 0.0003 4.388 | 1.605 × 102 | |
0.4596 |