Figure 6From: The role of the C8 proton of ATP in the catalysis of shikimate kinase and adenylate kinaseAdenylate kinase specific activity of WT, T39V and R97A mutant enzymes. The effect of the concentration of ATP and C8D-ATP on the specific activity of human adenylate kinase. ○ = WT AK1 using ATP, □ = WT AK1 using C8D-ATP, ● = T39V mutant using ATP, ■ = T39V mutant using C8D-ATP, ▲ = R97A mutant using ATP, Δ = R97A mutant using C8D-ATP. T39V and R97A data on right axis. Final enzyme concentrations were: WT AK: 0.33 mM, T39V: 2 nM and R97A: 20 nM. The assays were all run for 80 min.Back to article page