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Figure 6 | BMC Biochemistry

Figure 6

From: The role of the C8 proton of ATP in the catalysis of shikimate kinase and adenylate kinase

Figure 6

Adenylate kinase specific activity of WT, T39V and R97A mutant enzymes. The effect of the concentration of ATP and C8D-ATP on the specific activity of human adenylate kinase. = WT AK1 using ATP, □ = WT AK1 using C8D-ATP, = T39V mutant using ATP, ■ = T39V mutant using C8D-ATP, ▲ = R97A mutant using ATP, Δ = R97A mutant using C8D-ATP. T39V and R97A data on right axis. Final enzyme concentrations were: WT AK: 0.33 mM, T39V: 2 nM and R97A: 20 nM. The assays were all run for 80 min.

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