Figure 5

Confocal microscopy of myc-tagged DG constructs expressed in 293-Ebna cells. GFP and c-myc immunofluorescence staining of cells transfected with DG-A170-myc^GFPA), and DG-K500-myc^GFPB), constructs. GFP specific signal at 488 nm excitation, c-myc immunostaining at 543 nm excitation and their merge are shown. A XY confocal section is represented for each signal. A) and B) The GFP signal (green) shows that from both the DG-A170-myc^GFP and DG-K500-myc^GFP constructs originate a β-DG-GFP protein that localizes at the plasma membrane in a way reminiscent of the wild-type β-DG-GFP. A) C-myc immunostaining shows no plasma membrane signal in cells transfected with the DG-A170-myc^GFP construct, but only intense diffused and punctuated perinuclear (red) signals. B) The DG-K500-myc^GFP construct instead shows a strong plasma membrane localized c-myc immunostaining (red), whose localization is fully confirmed by the yellow-orange colour observable in the merged images.