Figure 4

Enrichment of the myc-tagged α-DG from total protein extracts. Immunoprecipitation of the myc-tagged α-DG (EL) with magnetic beads conjugated with an anti-myc antibody from a total protein extract of cells transfected with DG-K500-myc^GFP (TOT). A) Western blot was performed using an anti-myc-HRP conjugated antibody, which detects a band at 120 kDa. TOT, total protein extracts; NT, not transfected cells. B) Western blot performed using an anti β-DG antibody showing that α-DG-K500-myc still binds to β-DG since β-DG-GFP is found within the anti-myc pulled-down fraction. In cells transfected with DG-K500-myc^GFP , the band at 60 kDa corresponds to β-DG-GFP (black arrow), while in not transfected cells the band at 43 kDa (asterisk) corresponds to endogenous β-DG.