Figure 3

SDS-PAGE of S. flexneri LPS preparations of Oac mutants. LPS was subjected to Western blotting using MASF6 primary antibodies and anti-mouse IgG-HRP conjugated secondary antibodies. All samples contained approximately equal amount of LPS preparation as judged by preceding silver stained gels (gels not shown). SFL1899 is the positive control and SFL124 is the negative control. The Molecular mass marker used was the PageRuler^TM prestained protein ladder (Fermentas).