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Figure 6 | BMC Biochemistry

Figure 6

From: Quaternary structures of recombinant, cellular, and serum forms of Thymidine Kinase 1 from dogs and humans

Figure 6

Quaternary structures of serum TK1. (A) Thymidine kinase activity in serum fractions from dogs with acute lymphocytic leukemia (●) injected directly into the Superose 12 column, or pre-treated with 20 mM DTE (○). (B) Thymidine kinase activity in serum fractions from human patients with acute lymphocytic leukemia (♦) injected directly into Superose 12 or pre-treated with DTE (◊). Arrows indicate the elution position of molecular weight markers. (C) Western blot analyses of untreated dog serum samples (−DTE) or those pre-treated with 20 mM DTE (+DTE), using polyclonal anti-dog TK1 antibody. (D) Western blot analyses of untreated human serum samples (−DTE) or those pre-treated with DTE (+DTE), using monoclonal anti-human TK1 antibody. The numbers represent FPLC fractions.

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BMC Biochemistry

ISSN: 1471-2091

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