Table 1 The fluorescence emission intensity and λmax of Gtr1 and the mutations were measured as described in Methods
From: The intrinsic GTPase activity of the Gtr1 protein from Saccharomyces cerevisiae
Mutation | Relative intensity (GTPγS / GDP) | Emission spectra | |
|---|---|---|---|
λmax(GDP) | λmax (GTPγS) | ||
WT | 0.91 ± 0.01 | 329 ± 1 | 327 ± 1 |
Cys-less | 0.95 ± 0.02 | 327 ± 1 | 326 ± 1 |
Arg37Cys | 0.90 ± 0.01 | 325 ± 1 | 324 ± 1 |
Val67Cys | 0.96 ± 0.01 | 321 ± 2 | 316 ± 2 |