Analysis of binding modes, (SSB)
, of SSB
by FRET analysis. Protein-ssDNA binding and FRET assays were carried out as described in Materials & Methods . The FRET substrate was 40 nM 5′-Cy5-(dT)70-Cy3-3′. (a) Analysis of SSBEC binding to 5′-Cy5-(dT)70-Cy3-3′. Both (SSB)65 and (SSB)35 modes are clearly observed (22). (b) Analysis of SSBBA binding to 5′-Cy5-(dT)70-Cy3-3′. Only (SSB)65, not (SSB)35, mode was observed.