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Figure 9 | BMC Biochemistry

Figure 9

From: Thermodynamic analysis of DNA binding by a Bacillus single stranded DNA binding protein

Figure 9

Analysis of binding modes, (SSB) 35 and (SSB) 65 , of SSB EC and SSB BA by FRET analysis. Protein-ssDNA binding and FRET assays were carried out as described in Materials & Methods [26]. The FRET substrate was 40 nM 5′-Cy5-(dT)70-Cy3-3′. (a) Analysis of SSBEC binding to 5′-Cy5-(dT)70-Cy3-3′. Both (SSB)65 and (SSB)35 modes are clearly observed (22). (b) Analysis of SSBBA binding to 5′-Cy5-(dT)70-Cy3-3′. Only (SSB)65, not (SSB)35, mode was observed.

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