Temperature dependence of SSB
·ssDNA complex formation. Temperature dependence of ssDNA binding by SSBBA was measured at temperatures as indicated. (a) Binding isotherms for SSBBA binding to ssDNA at seven temperatures: 20°C, 25°C, 32°C and 37°C are shown. The concentration of the oligonucleotide was 1.0 nM and 25 mM NaCl was added. (b) Van’t Hoff plot was made using the analysis of the KD values obtained from the nonlinear regression of plot in 5A.