Figure 4

tyrHDL increases cellular ABCA1 in the presence of ALLN. Cholesterol-loaded fibroblasts were incubated with BSA and 10 μg/ml apoA-I, HDL, or tyrHDL with or without protease inhibitor ALLN (50 μM) to inhibit endogenous calpain for 16 h. ABCA1 was determined by immunoblotting as in Figure 3. The data are representative of three experiments with similar results.