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Figure 3 | BMC Biochemistry

Figure 3

From: Lipid-bound apolipoproteins in tyrosyl radical-oxidized HDL stabilize ABCA1 like lipid-free apolipoprotein A-I

Figure 3

tyrHDL protects ABCA1 from calpain-mediated degradation. Cholesterol-loaded fibroblasts pretreated with BSA plus 10 μg/ml apoA-I, HDL, or tyrHDL for 2 h or 16 h were then incubated with or without 0.5 μM μ-calpain following digitonin permeabilization. ABCA1 protein was analyzed by 5-15% SDS-PAGE gradient gels followed by immunoblotting. Results of duplicate incubations with apoA-I, HDL, and tyrHDL are shown. The data are representative of three experiments with similar results.

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BMC Biochemistry

ISSN: 1471-2091

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