Figure 5
Effect of B. breve MCRA expression on survivability of L. lactis and C. glutamicum strains under stress conditions. A) Survivability of L. lactis pNZ8048 ('black square') versus pEMYL1 ('upside down black triangle') following heat treatment at 51°C for 40 min. ** Denotes value significantly different (p = 0.01) than control (pNZ8048). B) Survivability of C. glutamicum pCLIK ('black square') versus pEMYC2 ('black triangle') following heat treatment at 51°C for 30 min * Denotes value significantly different (p = 0.05) than control (C. glutamicum pCLIK). C) Survivability of C. glutamicum pCLIK ('upside down black triangle') versus pEMYC2 ('black square') following growth in 1-5% (v/v) butanol for 1 h. D) Survivability of L. lactis pNZ8048 ('black triangle') versus pEMYL1 ('black square') following nisin induction for 1.5 h prior to growth in 3% (v/v) butanol for 1 h. The experiments were done at least in triplicate. * Denotes value significantly different (p = 0.05) than control (pNZ8048). E) Survivability of C. glutamicum pCLIK ('upside down black triangle')versus pEMYC2 ('black square') following growth in 3% (v/v) butanol for 45 min. F) Survivability of C. glutamicum pCLIK ('black triangle')versus pEMYC2 ('black square') following growth in 4.8 M NaCl for 90 min. G) Survivability of L. lactis pNZ8048 ('black triangle') versus pEMYL1 ('black square') following nisin induction for 1.5 h prior to growth in 3 M NaCl for 90 min. The experiments were done in triplicate.