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Figure 2 | BMC Biochemistry

Figure 2

From: Myosin-cross-reactive antigen (MCRA) protein from Bifidobacterium breve is a FAD-dependent fatty acid hydratase which has a function in stress protection

Figure 2

Spectral analysis of purified B. breve MCRA protein. SDS PAGE analysis of B. breve MCRA protein purified on Ni-NTA column. Lane 1, molecular weight marker, lane 2, B. breve MCRA protein of apparent MW of 66 kDa, lane 3, immunoblot with anti-His tag antibody shows the absence of significant proteolytic degradation in purified B. breve MCRA. B UV-Vis spectrum of B. breve MCRA protein (black) and the supernatant after heat precipitation (red), showing flavin-like absorbance. For the reference spectra of 10 ÎĽM FAD (blue) and FMN (magenta) are shown.

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BMC Biochemistry

ISSN: 1471-2091

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