Skip to main content
Figure 4 | BMC Biochemistry

Figure 4

From: Protein kinase A type I activates a CRE-element more efficiently than protein kinase A type II regardless of C subunit isoform

Figure 4

Expressed RIα and RIIα inhibit Cα1- and Cβ2-dependent CREB phosphorylation in a dose-dependent manner. 293T cells were co-transfected with increasing amounts (0-1280 ng) of either pDeRIα (A and B) or pExRIIα (C and D) and a fixed amount of pDeCα1 (300 ng, A and C) or pDeCβ2 (1400 ng, B and D). Cells were harvested after 24 hours and cell extracts adjusted to 1 mg total protein/mL and assayed at 560 nm for PKA-specific phosphorylation of CREB measured as CRE-activity. Data points represent relative luciferase activity +/- SD (n = 2-6).

Back to article page

BMC Biochemistry

ISSN: 1471-2091

Contact us