Skip to main content
Figure 3 | BMC Biochemistry

Figure 3

From: Protein kinase A type I activates a CRE-element more efficiently than protein kinase A type II regardless of C subunit isoform

Figure 3

Expressed RIα and RIIα inhibit expressed Cα1 and Cβ2 catalytic activity in a dose-dependent manner. 293T cells were co-transfected with increasing amounts (0-1280 ng) of either pDeRIα (A and B) or pExRIIα (C and D) with a fixed amount of pDeCα1 (300 ng, A and C) or pDeCβ2 (1400 ng, B and D). Cells were harvested after 24 hours, cell extracts adjusted to 1 mg total protein/mL and assayed for PKA-specific phosphotransferase activity in the presence (+ cAMP) and absence (- cAMP) of 7.14 μM cAMP. Data points represent pmol ATP transferred/min/mg protein) +/- SD (n = 2-6).

Back to article page