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Figure 1 | BMC Biochemistry

Figure 1

From: Protein kinase A type I activates a CRE-element more efficiently than protein kinase A type II regardless of C subunit isoform

Figure 1

PKAI (RIαCα1) and PKAII (RIIαCα1) are expressed in 293T cells. (A) Cell extracts of 293T cells (40 μg protein/lane) were analyzed by immunoblotting using a pan-anti-C antibody (upper left panel) and anti-RIα and anti-RIIα (lower left panels). The levels and identities of 293T cell C and R subunit expression were compared to human peripheral blood lymphocytes (hPBL) revealing expression of Cα1, RIα and RIIα. No detectable levels of RIβ and RIIβ were identified when compared to extracts of human temporal cortex (hTempCortex, right panels). (B) Immunofluorescence analysis of PKA RI and RII in 293T cells. RIα (Anti-RI, red) is expressed diffusely in the cytosol and RIIα (Anti-RII, green) is expressed in the Golgi-centrosomal area of 293T cells. (C) Immunofluorescence analysis of PKA C subunits in 293T treated without (-) or with (+) 340 μM 8-CPT-cAMP.

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BMC Biochemistry

ISSN: 1471-2091

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