Topological characterisation and identification of critical domains within glucosyltransferase IV (GtrIV) of Shigella flexneri

Table 2 Predicted structures of the four chimeric proteins created in this study between GtrIc and GtrIV

Points of fusion: a) GtrIc-GtrIV loop No.2-GtrIc chimera:GtrIc-F31/GtrIV-N27, GtrIV-Y68/GtrIc-V100, b) GtrIV-GtrIc loop No.2-GtrIV chimera: GtrIV-M26/GtrIc-H32, GtrIc-N99/GtrIV-S71, c) GtrIc-GtrIV loop No.6-GtrIc chimera: GtrIc-P382/GtrIV-T231, GtrIV-K351/GtrIc-V495, d) GtrIV-GtrIc loop No.10-GtrIV chimera: GtrIV-S229/GtrIc-T231, GtrIc-V381/GtrIV-D352. To test for GtrIc function, the chimera constructs were transformed in to SFL1416 (Serotype 1a) to test for serotype conversion to serotype 1c using MASF Ic. As for GtrIV function, conversion to serotype 4a was tested by using Type IV antisera against constructs that were transformed into SFL1616 (serotype Y). All membrane protein westerns were carried using anti-alkaline phosphatse antibodies.

ISSN: 1471-2091