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Figure 1 | BMC Biochemistry

Figure 1

From: Topological characterisation and identification of critical domains within glucosyltransferase IV (GtrIV) of Shigella flexneri

Figure 1

The final GtrIV topology model. Final GtrIV topology model after the creation of gtrIV-phoA/lacZ fusions by the Exo III deletion and PCR-based approaches, and gtrIV-phoA/lacZ-gtrIV sandwich fusions. In this model, GtrIV is shown to have 8 transmembrane helices and two large periplasmic loops. All Exo III mediated fusions are indicated with the closed circles while PCR mediated fusions are indicated with the crosses. All sandwich fusions are indicated with the stars. Blue coloured stars, circles and crosses depict fusions that have high PhoA NARs and appear blue on DI plates. Similarly, the ones in red depict fusions that have high LacZ NARs and appear red on DI plates. All purple fusions are seen to have occurred within transmembrane helices. The proposed re-entrant loop which traverses the plasma membrane from the periplasm into the cytoplasm, forming a cytoplasmic loop, occurs between transmembrane helices III and IV. All fusions have been labelled with the residue at which the fusion with PhoA/LacZ takes place or, in the case of sandwich fusions, where the dual reporter protein has been inserted.

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