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Figure 6 | BMC Biochemistry

Figure 6

From: Low affinity PEGylated hemoglobin from Trematomus bernacchii, a model for hemoglobin-based blood substitutes

Figure 6

NO dioxygenase reactivity. Determination of the effect of PEGylation on NO dioxygenase reactivity of HbA and Tb Hb and their PEGylated derivatives. The reaction was carried out by rapid mixing under anaerobic conditions at 20°C. 3 μM oxygenated HbA (gray dotted line), Tb Hb (gray solid line), PEG-Hboxy (black dotted line), and PEGTb Hb (black solid line), with a deoxygenated PBS solution containing 12 μM NO. The reaction was monitored at 405 nm and absorbance differences were normalized.

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