Figure 4

Oxygen binding properties of derivatized hemoglobins. Hill plots of oxygen-binding curves of HbA (closed squares), Tb Hb (closed circles) and PEGTb Hb (open circles), measured in 100 mM HEPES 1 mM EDTA, 5 mM sodium ascorbate, 10³ U/ml catalase, pH 7.0, at 10°C. Experimental points are fitted to the Hill equation, with calculated Hill's coefficients and P₅₀'s reported in Table 1.