Cofactor requirement of FAR enzymes. (a) Cofactor requirement of TaFAR1 and TaFAR2. Assays were carried out with the total membrane fraction of yeast cells expressing TaFAR1 or TaFAR2. [1-14C]-labeled products derived from assays carried out with labeled 16:0-CoA (lane 1 and 2, TaFAR1) or labeled 18:0-CoA (lane 3 and 4, TaFAR2) and NADH (lane 1 and 3) or NADPH (lane 2 and 4). (ALD: fatty aldehyde, FFA: free fatty acid, R-OH: fatty alcohol). (b) NADPH dependency of TaFAR1. Assays were conducted with the total membrane fraction of yeast cells expressing TaFAR1 and with [1-14C]-labeled 16:0-CoA. The concentration of NADPH was varied under otherwise standard assay conditions. Results are the sum of synthesized aldehydes and alcohols and display mean values of two independent assays.