Figure 2
Alcohol production of yeast strains expressing avian FAR enzymes from endogenous fatty acids. (a) TLC analysis of lipid extracts from transgenic yeast cells. Total lipid fraction of yeast cells expressing one of the avian FAR sequences or the empty vector control were extracted after 72 h incubation and lipid extracts were analyzed by TLC. FFA: free fatty acids, R-OH: fatty alcohols. (b) Alcohol composition of transgenic yeast strains. The alcohol composition was analyzed and quantified by GC. Results are mean values of duplicate extractions from two independent yeast cultures. Following concentrations in μmol*g-1 fresh weight correspond to 100%: AdFAR1:15.6, GgFAR1:13.2, TaFAR1: 17.7, GgFAR2: 8.2, TaFAR2: 2.9, control: 0.02. Because GgFAR1db showed results very similar to those of GgFAR1, results of GgFAR1 are presented only.