Figure 6

Characterization of glycosylated endoproteinase Glu-C digestion products of LMW Dsp. A: Contents of fraction AS2R-g characterized by SDS-PAGE stained with CBB (1), stained with stains-all (2); by Western blotting using the Dsp antibody (3) or Dgp antibody (4). B: RP-HPLC chromatogram (Abs²²⁰ v. min) of endoproteinase Glu-C digestion products of AS2R-g. C: Table showing the results of mass spectrometric (LC/MSMS) analyses of RP-HPLC fractions following glycopeptidase digestion. Asparagines (N) in the appropriate context for N-glycosylation that were also found to be 1 Da smaller than expected (due to deamidation) are indicated in bold and listed as the posttranslational modification (PTM) site on the right.