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Figure 4 | BMC Biochemistry

Figure 4

From: Porcine dentin sialoprotein glycosylation and glycosaminoglycan attachments

Figure 4

Characterization of disaccharides attached at Ser238 and Ser250. Supelcosil LC-NH2 chromatograms (Abs232 v. min) of GAG products released from ANS1/2R3-Prp (Ser238) and ANS1/2R3-Prd (Ser250) by chondroitinase ABC digestion compared to GAG standards (ΔDi-0S; ΔDi-6S; ΔDi-4S; ΔDi-diSD; ΔDi-diSE; ΔDi-triS). Based upon their retention times, the GAG attachments are observed to contain chondroitin 6-sulfate (ΔDi-6S) and chondroitin 4-sulfate (ΔDi-4S). Integration of the areas under the peaks show the ratio of C6 S to C4 S is 7 to 3.

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