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Figure 1 | BMC Biochemistry

Figure 1

From: Porcine dentin sialoprotein glycosylation and glycosaminoglycan attachments

Figure 1

Dentin power extraction diagram. The extracts, in bold, are the first guanidine extract (G1), first guanidine pellet (G1P), acid (A), acid salt (AN), Tris salt (TN), residual insoluble material (RIM), second guanidine extract (G2), and the second guanidine pellet (G2P). Selected extractions were fractionated to isolate Dspp cleavage products. The AN extract was fractionated by size exclusion (S) and RP-HPLC (R) to yield HMW Dsp in fractions ANS1/2-R3, ANS1/2-R4, and ANS1/2-R5. The A extract was fractionated by size exclusion and RP-HPLC to yield LMW Dsp (AS2R-g). Chondroitinase activity was detected in the second ion exchange (Q) fraction of the G2 extact (G2Q2).

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