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Table 1 Summary of results obtained from thermal shift assays for the identification of GbpA- and HbpA2-family ligands out of a test set of typically family 5 SBP allocrites.

From: Delineation of the Pasteurellaceae-specific GbpA-family of glutathione-binding proteins

 

GbpAHi

GbpAAp

GbpAHp

GbpAPm

HbpA2Hp

HbpA2Ap

 

T m (°C) a

GSSG

17.5

14.0

16.0

20.5

-

-

GSH

12.5

11.0

14.0

13.0

-

-

S-methylglutathione

10.5

7.5

11.5

11.5

-

-

S-nitrosoglutathione

7.0

6.5

9.5

12.0

-

-

glutathione cysteine disulfide

10.0

6.0

7.0

12.0

-

-

S-hexylglutathione

-

-

4.5

6.5

-

-

S-decylglutathione

-

-

2.5

3.0

-

-

homoglutathione

γ-glutamylcysteine

cysteinylglycine

glycylleucine

glycylglycylcysteine

δ-aminolevulinic acid

proline-betaine

nickel}

-

-

-

-

-

-

  1. aInteracting ligands are identified by means of the respective ΔTm-values, the apparent Tm-differences in °C for the ligand-protein complexes relative to the uncomplexed proteins. A negative sign indicates ligands for which the ΔTm is smaller than 1.5°C.