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Figure 4 | BMC Biochemistry

Figure 4

From: Delineation of the Pasteurellaceae-specific GbpA-family of glutathione-binding proteins

Figure 4

Determination of the affinity constant of GbpA Hp for three different glutathione forms: the oxidized (GSSG), the reduced (GSH), and an S-derivatized form (S-me-GSH). Isothermal titration data for the titration of GbpAHp with either GSSG, GSH, or S-me-GSH in 10 mMTris-HCl, pH 7.4. The upper panels show the calorimetric titrations for 10-μl injections with 300 s between injections. The lower panels represent the integrated heat values (from the upper panels) as a function of the protein/ligand molar ratio in the cell. The solid line represents the best fit of the single-site model to the experimental points.

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