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Table 4 Oligonucleotide primers.

From: Three genes expressing Kunitz domains in the epididymis are related to genes of WFDC-type protease inhibitors and semen coagulum proteins in spite of lacking similarity between their protein products

Target gene

Purpose

Primer sequence (5' to 3')

Human SPINT3

RT-PCR and 3' RACE

CCTCTCTCTCGTTTCTCCTGATTCTCACT

Human SPINT3

RT-PCR and 5' RACE

CGCAGCCTCCGTAAGCAAATAACTCACAT

Human SPINT3

qRT-PCR

TCAGGCCTCTCTCTCGTTTCTCCTGATT

Human SPINT3

qRT-PCR

CCCTTTTCCATAGGAAAAGCGCATACAT

Human SPINT4

RT-PCR and 3' RACE

CATCTTCTGCTCATTGAATACCCTGTTATT

Human SPINT4

RT-PCR and 5' RACE

TTACAGCCGGAGAAGACAAAAGTTTCACAT

Human SPINT4

qRT-PCR

TCTTCTGCTCATTGAATACCCTGTTATT

Human SPINT4

qRT-PCR

GAACTTCATAGCAGCTTCCAAAATTCAT

Human SPINT5

3' RACE

CAACTATCCTCTGAAGAAGGGCACCTGT

Human SPINT5

Nested 3' RACE

ATGTGAGCCCTTTGTCTTCAGTGGCTGT

Human SPINT5

5' RACE

GTTTCTGTTGCCTCCACAGCCACTG

Human SPINT5

Nested 5' RACE

CAGCCACTGAAGACAAAGGGCTCAC

Human SPINT5

qRT-PCR

GCTGTGGAGGCAACAGAAACAACTTTAA

Human SPINT5

qRT-PCR

CCTCCTTCTGTCATATTAAGGCCCTATT

Mouse Spint5

RT-PCR and 3' RACE

CCTGCAGGAGTCCGAAGTCAACTCTGCGA

Mouse Spint5

RT-PCR and 5' RACE

TGCAACGGACTTCACAGAGGTATTTAGT

Human APRT

qRT-PCR

CTTGGACTGGGCTGCGTGCTCATCCGAA

Human APRT

qRT-PCR

GTCCTGGCTCCAGGGCGTCTTTCTGAAT

Human CSTB

qRT-PCR

AGTTCCCTGTGTTTAAGGCCGTGTCATT

Human CSTB

qRT-PCR

GTTTTCATGAGGGAGAGATTGGAACACT

Human SPINT3

Cloning in pET32b

GGATCCGAATTCG CGAGACACTATCAAGGATCTCCTCCCAAAT

Human SPINT3

Cloning in pET32b

TCGAGTGCGGCCGCTCA GGTGAACTTGCAGAATTTCTCACAT

Human SPINT4

Cloning in pET32b

CGAATTCG GGTGGTGTTAATAAAATTGCGGAGA

Human SPINT4

Cloning in pET32b

GCGCGGCCGC CTATTGACTTTGGTCCTTTTCAG

Human SPINT5

Cloning in pET32b

CAGTGAATTCCG AATACTGCAACTATCCTCTGAAGAA

Human SPINT5

Cloning in pET32b

TGCAGCGGCCGCTA CTTTTCAGTAATACACATTTTTTCACAGA

Bovine BPTI

Cloning in pET32b

GGATCCGAATTCG CCTGACTTCTGCCTAGAGCCTCCATAT

Bovine BPTI

Cloning in pET32b

TCGAGTGCGGCCGCTCA CCAGGGCCCAATAGCACCACCACAGGT

  1. The underlined sequences are not present in the target genes.