BMC Biochemistry

Table 3 Tissue specific expression.

From: Three genes expressing Kunitz domains in the epididymis are related to genes of WFDC-type protease inhibitors and semen coagulum proteins in spite of lacking similarity between their protein products

		Relative transcript levels (%)
		Gene used for normalization
Tissue	Gene	APRT	σ	CSTB	σ
Breast	SPINT3	0,010	0,001	0,018	0,002
	SPINT4	0,006	0,002	0,010	0,004
Epididymis	SPINT3	45	5	45	7
	SPINT4	100	14	100	18
	SPINT5	0,003	0,0005	0,003	0,0006
Kidney	SPINT3	0,0006	0,0002	0,002	0,0004
	SPINT4	0.006	0,002	0,016	0,002
Prostate	SPINT3	0,0007	0,0005	0,001	0,0007
	SPINT4	0,001	0,001	0,002	0,002
Seminal vesicle	SPINT3	0,056	0,017	0,053	0,014
	SPINT4	0,015	0,004	0,014	0,003
Skin	SPINT3	0,029	0,007	0,053	0,013
	SPINT4	0,003	0,001	0,005	0,002
Thyroid	SPINT3	0,004	0,003	0,005	0,004
	SPINT4	0,012	0,004	0,013	0,004
Testis	SPINT3	No signal	No signal	No signal	No signal
	SPINT4	0,004	0,004	0,008	0,007

Transcript levels of putative Kunitz inhibitors in normal tissues were analyzed by qRT-PCR. The transcript levels were calculated by the comparative ΔΔCt method and normalized with the housekeeping genes APRT and CSTB. Transcript levels are given as % of SPINT4 levels in the epididymis, which was set to 100%. SPINT5 was only detected in the epididymis.

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ISSN: 1471-2091

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