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Table 2 PCR efficiency

From: Three genes expressing Kunitz domains in the epididymis are related to genes of WFDC-type protease inhibitors and semen coagulum proteins in spite of lacking similarity between their protein products

Gene

Ct value range

Slope

R2

PCR efficiency

APRT

23-31

-3.14

0.996

2.08

CSTB

20-29

-3.35

0.996

1.99

SPINT3

20-29

-3.26

0.998

2.02

SPINT4

21-28

-3.48

0.997

1.94

  1. The efficiency of PCR experiments was calculated by regression analysis of Ct values generated by serial dilution of epididymis cDNA.
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BMC Biochemistry

ISSN: 1471-2091

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