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Figure 4 | BMC Biochemistry

Figure 4

From: Three genes expressing Kunitz domains in the epididymis are related to genes of WFDC-type protease inhibitors and semen coagulum proteins in spite of lacking similarity between their protein products

Figure 4

Protease inhibition with recombinant BPTI. (A) Serial dilutions of recombinant BPTI were incubated with 20 nM bovine chymotrypsin (■), 50 nM human plasmin (▼), and 5 nM bovine trypsin (▲). (B) Recombinant BPTI (filled symbols) and BPTI isolated from bovine pancreas (open symbols) were incubated with 10 nM (circles) and 20 nM (squares) bovine chymotrypsin. Residual amidolytic activity was measured and compared with samples without added inhibitor

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BMC Biochemistry

ISSN: 1471-2091

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